Performance of BD Max StaphSR for Screening of Methicillin-Resistant Staphylococcus aureus Isolates among a Contemporary and Diverse Collection from 146 Institutions Located in Nine U.S. Census Regions: Prevalence of mecA Dropout Mutants.
نویسندگان
چکیده
This study determined the performance of BD Max StaphSR and the rate of methicillin-resistant Staphylococcus aureus (MRSA) with an unrecognized staphylococcal cassette chromosome mec (SCCmec) right-extremity junction (MREJ) region among 907 methicillin-resistant S. aureus (MRSA) and 900 methicillin-susceptible S. aureus (MSSA) isolates. The rate of mecA/mecC dropout mutants was also evaluated. Only three MRSA isolates (99.7% sensitivity; 904/907) were classified as MSSA by the BD Max StaphSR assay, due to negative results for MREJ. Eight MSSA isolates (99.1% sensitivity; 892/900) were assigned as MRSA. However, six of these MSSA isolates had the mecA gene confirmed by PCR and sequencing (99.8% sensitivity; 898/900). Overall, 7.1% (64/900) of MSSA isolates showed results compatible with a mecA dropout genotype.
منابع مشابه
Comparison of BD Max StaphSR and BD Max MRSAXT for Screening of Staphylococcus aureus Clinical Isolates Collected from Hospitals in the United States.
We recently reported the performance of the BD Max StaphSR assay for screening of methicillin-resistant Staphylococcus aureus (MRSA), as well as the prevalence in the U.S. of S. aureus carrying a staphylococcal cassette chromosome (SCCmec) element lacking mecA (so-called dropout mutant) (1). This assay targets the nuc and mecA/C genes and 11 sequences of the SCCmec-orfX right-extremity junction...
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ورودعنوان ژورنال:
- Journal of clinical microbiology
دوره 54 1 شماره
صفحات -
تاریخ انتشار 2016