Performance of BD Max StaphSR for Screening of Methicillin-Resistant Staphylococcus aureus Isolates among a Contemporary and Diverse Collection from 146 Institutions Located in Nine U.S. Census Regions: Prevalence of mecA Dropout Mutants.

نویسندگان

  • Rodrigo E Mendes
  • Amy A Watters
  • Paul R Rhomberg
  • David J Farrell
  • Ronald N Jones
چکیده

This study determined the performance of BD Max StaphSR and the rate of methicillin-resistant Staphylococcus aureus (MRSA) with an unrecognized staphylococcal cassette chromosome mec (SCCmec) right-extremity junction (MREJ) region among 907 methicillin-resistant S. aureus (MRSA) and 900 methicillin-susceptible S. aureus (MSSA) isolates. The rate of mecA/mecC dropout mutants was also evaluated. Only three MRSA isolates (99.7% sensitivity; 904/907) were classified as MSSA by the BD Max StaphSR assay, due to negative results for MREJ. Eight MSSA isolates (99.1% sensitivity; 892/900) were assigned as MRSA. However, six of these MSSA isolates had the mecA gene confirmed by PCR and sequencing (99.8% sensitivity; 898/900). Overall, 7.1% (64/900) of MSSA isolates showed results compatible with a mecA dropout genotype.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Comparison of BD Max StaphSR and BD Max MRSAXT for Screening of Staphylococcus aureus Clinical Isolates Collected from Hospitals in the United States.

We recently reported the performance of the BD Max StaphSR assay for screening of methicillin-resistant Staphylococcus aureus (MRSA), as well as the prevalence in the U.S. of S. aureus carrying a staphylococcal cassette chromosome (SCCmec) element lacking mecA (so-called dropout mutant) (1). This assay targets the nuc and mecA/C genes and 11 sequences of the SCCmec-orfX right-extremity junction...

متن کامل

Detection of Methicillin-resistant Staphylococcus aureus according to mecA and femA Genes Among Hospitalized Patients in Kashan and Isfahan Hospitals

Background and Aim: Increased prevalence of methicillin-resistant Staphylococcus aureus (MRSA) and treatment of infections due to these resistant strains is one of the most important health challenges. In this study, antimicrobial resistance patterns of Staphylococcus aureus isolates from patients were determined to commonly prescribed and last-resort antibiotics, and methicillin-resistant Stap...

متن کامل

Detection of Methicillin-Resistant Staphylococcus Aureus by Phenotypical and Molecular Methods among Clinical Isolates

Abstract Background and Objective: Increasing prevalence of methicillin resistant Staphylococcus aureus strains (MRSA) with their multidrug resistance potential causes difficulties in the treatment of infections due to these bacteria. Hence, the detection and determination of the frequency of MRSA strains via phenotypical and molecular methods is necessary in different parts of the county. ...

متن کامل

Prevalence of mecA Gene of Methicillin Resistant Staphylococcus spp. Isolated from Nosocomial Infections and Environmental Specimens in Sanandaj Hospitals, Kurdistan, Iran

Background: Methicillin resistant Staphylococcus aureus (MRSA) is one of the major agents for increasing number of serious hospital and community acquired infections. The aim of this study was to investigate the occurrence of the MRSA and mecA gene among nosocomial and environmental specimens in Kurdistan hospitals and determining the antibiotic resistance of the isolates. Materials and Method...

متن کامل

Prevalence of resistance and toxin genes in community-acquired and hospital-acquired methicillin-resistant Staphylococcus aureus clinical isolates

Objective(s): Methicillin-resistant Staphylococcus aureus (MRSA) is one of the major health hazards and became of greater public health concern since the emergence of community-acquired MRSA. This work aimed to study the prevalence of mecA, femA, femB, lukS-PV, lukF-PV (PVL), intI, and intII genes among community-acquired (CA) hospital-acquired (HA) MRSA to increase vi...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Journal of clinical microbiology

دوره 54 1  شماره 

صفحات  -

تاریخ انتشار 2016